What is the primary enzyme responsible for unwinding the DNA double helix during replication, separating the two strands?

✓ Correct Answer: Option C

Helicase is the enzyme that unwinds the DNA double helix by breaking the hydrogen bonds between complementary base pairs, allowing the replication fork to advance. DNA Polymerase synthesizes new DNA strands, Ligase joins DNA fragments, and Primase synthesizes RNA primers.