💊 PCAT Exam Prep

Thin-layer chromatography (TLC) separates compounds based on:

A Size only
B Differential affinity for the stationary phase (silica) vs. the mobile phase (solvent)
C Charge only
D Molecular weight only

✓ Correct Answer: Option B

TLC: polar compounds have higher affinity for silica (low Rf). Nonpolar compounds travel further with nonpolar solvent (higher Rf).

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