Which enzyme is considered the rate-limiting step in gluconeogenesis?

A Hexokinase

B Phosphofructokinase-1 (PFK-1)

C Pyruvate carboxylase

D Fructose-1,6-bisphosphatase

✓ Correct Answer: Option D

Fructose-1,6-bisphosphatase is the key regulatory and rate-limiting enzyme in gluconeogenesis. It catalyzes the irreversible conversion of fructose-1,6-bisphosphate to fructose-6-phosphate, bypassing the PFK-1 step of glycolysis.

💡 Why is D) the correct answer?

The correct answer to the question "Which enzyme is considered the rate-limiting step in gluconeogenesis?" is Option D: Fructose-1,6-bisphosphatase.

The other options — A) Hexokinase, B) Phosphofructokinase-1 (PFK-1), C) Pyruvate carboxylase — are incorrect for this USMLE Exam Prep question. As explained above: Fructose-1,6-bisphosphatase is the key regulatory and rate-limiting enzyme in gluconeogenesis. It catalyzes the irreversible conversion of fructose-1,6-bisphosphate to fructose-6-phosphate, bypassing…

📚 About This USMLE Exam Prep Practice Question

This 2-level USMLE Exam Prep question from the Step 1 – Basic Sciences topic is part of BrainForge Quiz's free exam preparation library. Each question includes the correct answer and a detailed explanation to help you understand the underlying concept, not just memorize the answer.

How to use this page: Read the question above, consider all four options (A through D), then check the highlighted correct answer and explanation. For active timed practice, start a free USMLE Exam Prep practice test or use flashcards for spaced repetition review.

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Which enzyme is considered the rate-limiting step in gluconeogenesis?

✓ Correct Answer: Option D

📚 About This USMLE Exam Prep Practice Question

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