Polymerase Chain Reaction (PCR) requires several components for successful amplification of DNA. Which of the following is essential for defining the specific region of DNA to be amplified?

✓ Correct Answer: Option C

Primers are short, synthetic DNA oligonucleotides that bind to complementary sequences flanking the target region. They provide a free 3'-hydroxyl group for Taq polymerase to extend from, thus defining the amplified segment.